A Comparative Study of the Effects of Hybrid and Hyaluronic Acid Fillers on Secondary Intention Wound Healing

Section: Research Paper
Issue
Vol. 25 No. 2 (2025): Volume 25 Issue 2
Pages
271-286

Abstract

Aims: To compare the effects of Hybrid filler (hyaluronic acid + calcium hydroxylapatite) and hyaluronic acid filler (HA) on skin healing after induced wounds using histological analysis. Materials and Methods: Three groups of three male Albino rats were randomly placed in the 3rd, 7th, and 14th repair times. Rats have three circular cuts on their backs. The top wound got hybrid filler (NEAUVIA STIMULATE), the bottom right wound got hyaluronic acid filler (TEOSYAL RHA 3), and the bottom left wound wasn't filled as a test. On days 3, 7, and 14, biopsies were taken from each rat and sent to a pathologist for histopathology. Results: The treated groups showed less inflammation than the control group, especially on the third and seventh days. On the fourteenth day, the tested groups had much lower granulation tissue production rates than the control group, which stayed high. On day 14, the hybrid filler group had the most re-epithelialization. Conclusion: The skin of rats healed faster with hybrid and HA fillers. They may help wounds heal.

Keywords:

Hybrid Filler, HA Filler, Secondary Wound Healing

References

  1. Mendonça RJd, Coutinho-Netto J. Cellular aspects of wound healing. Anais brasileiros de dermatologia. 2009;84:257-262.
  2. Baviskar K, Shaikh T, Patil K, Baviskar R, Lodhi S. Role of hyaluronic acid based hydrogel in management of wound healing effect. Advance Pharmaceutical Journal. 01/01 2019;4:152-160.
  3. Celani LMS, Lopes IS, Medeiros AC. The effect of hyaluronic acid on the skin healing in rats. JOURNAL OF SURGICAL AND CLINICAL RESEARCH. 2019;10(2):65-75.
  4. Shaharudin A, Aziz Z. Effectiveness of hyaluronic acid and its derivatives on chronic wounds: a systematic review. Journal of wound care. 2016;25(10):585-592.
  5. Pierre S, Liew S, Bernardin A. Basics of dermal filler rheology. Dermatologic surgery. 2015;41:S120-S126.
  6. Chang JW, Koo WY, Kim E-K, Lee SW, Lee JH. Facial rejuvenation using a mixture of calcium hydroxylapatite filler and hyaluronic acid filler. Journal of Craniofacial Surgery. 2020;31(1):e18-e21.
  7. Moradi A, Shirazi A, David R. Nonsurgical chin and jawline augmentation using calcium hydroxylapatite and hyaluronic acid fillers. Facial Plastic Surgery. 2019;35(02):140-148.
  8. Koksal V, Mercantepe T, Tumkaya L, Oktem IS. Less Use of the Bipolar Cautery can Prevent Postlaminectomy Epidural Fibrosis: An Experimental Study in Rats. Turkish Neurosurgery. 2019;30(2).
  9. Camacho-Alonso F, López-Jornet P. Clinical-pathological study of the healing of wounds provoked on the dorso-lingual mucosa in 186 albino rats. Otolaryngology—Head and Neck Surgery. 2007;136(1):119-124.
  10. Parikh KS. Nano-structured, drug-eluting medical devices for improved clinical outcomes, Johns Hopkins University; 2017.
  11. Huh J-B, Kim J-H, Kim S, et al. Effects of PMMA and cross-linked dextran filler for soft tissue augmentation in rats. International Journal of Molecular Sciences. 2015;16(12):28523-28533.
  12. Sultana J, Molla MR, Kamal M, Shahidullah M, Begum F, Bashar MA. Histological differences in wound healing in maxillofacial region in patients with or without risk factors. Bangladesh Journal of Pathology. 2009;24(1):3-8.
  13. Gupta A, Kumar P. Assessment of the histological state of the healing wound. Plastic and Aesthetic Research. 2015;2:239-242.
  14. Sinha UK, Gallagher LA. Effects of steel scalpel, ultrasonic scalpel, CO2 laser, and monopolar and bipolar electrosurgery on wound healing in guinea pig oral mucosa. The Laryngoscope. 2003;113(2):228-236.
  15. Masson‐Meyers DS, Andrade TA, Caetano GF, et al. Experimental models and methods for cutaneous wound healing assessment. International journal of experimental pathology. 2020;101(1-2):21-37.
  16. Schilrreff P, Alexiev U. Chronic inflammation in non-healing skin wounds and promising natural bioactive compounds treatment. International journal of molecular sciences. 2022;23(9):4928.
  17. Khalaf AA, Hassanen EI, Zaki AR, Tohamy AF, Ibrahim MA. Histopathological, immunohistochemical, and molecular studies for determination of wound age and vitality in rats. International Wound Journal. 2019;16(6):1416-1425.
  18. Trombelli L, Simonelli A, Pramstraller M, et al. Clinical efficacy of a chlorhexidine‐based mouthrinse containing hyaluronic acid and an antidiscoloration system in patients undergoing flap surgery: a triple‐blind, parallel‐arm, randomized controlled trial. International Journal of Dental Hygiene. 2018;16(4):541-552.
  19. Urdiales‐Gálvez F, Braz A, Cavallini M. Facial rejuvenation with the new hybrid filler HArmonyCa™: Clinical and aesthetic outcomes assessed by 2D and 3D photographs, ultrasound, and elastography. Journal of Cosmetic Dermatology. 2023.
  20. Kawano Y, Patrulea V, Sublet E, et al. Wound healing promotion by hyaluronic acid: Effect of molecular weight on gene expression and in vivo wound closure. Pharmaceuticals. 2021;14(4):301.
  21. Kim J. Multilayered injection of calcium hydroxylapatite filler on ischial soft tissue to rejuvenate the previous phase of chronic sitting pressure sore. Clinical, Cosmetic and Investigational Dermatology. 2019:771-784.
  22. Marinho A, Nunes C, Reis S. Hyaluronic acid: a key ingredient in the therapy of inflammation. Biomolecules. 2021;11(10):1518.
  23. Larkina S, Seletska O, Makarenko O, Vastyanov R, Badiuk N. Study of osteoplastic and anti-inflammatory efficiency of drugs containing calcium hydroxyapatite and hyaluronic acid. 2021.
  24. Gao Y, Sun Y, Yang H, et al. A low molecular weight hyaluronic acid derivative accelerates excisional wound healing by modulating pro-inflammation, promoting epithelialization and neovascularization, and remodeling collagen. International journal of molecular sciences. 2019;20(15):3722.
  25. Hameed MA, Abbas MK, Madlum KN. Role of hyaluronic acid in modulating wound healing progression. Systematic Reviews in Pharmacy. 2020;11(11):1844-1850.

Authors

Mohammed Nadhem Mustafa

Oral and Maxillofacial Surgery Department/College of Dentistry/University of Mosul

Abdulhameed N. Aldabagh

Oral and Maxillofacial Surgery Department/College of Dentistry/University of Mosul, Iraq

Abdulsattar Salim Mahmood

Oral Diagnosis Department/College of Dentistry/University of Mosul, Iraq

Identifiers

DOI: 10.33899/rdenj.2023.143099.1222

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