Remineralization Efficacy of Grape Seed Oil Paste on Demineralized Enamel after Bracket Debonding Compared to Casein Phosphopeptide-Amorphous Calcium Fluoride Phosphate Paste: An In Vitro Study

Section: Research Paper
Issue
Vol. 26 No. 1 (2026): Volume 26 Issue 1
Pages
19-37

Keywords:

Grape seed oil, CPP-ACPF, Debonding, Demineralization, Remineralization.

Abstract

Aims: The present study aimed to evaluate the remineralization potential of grape seed (GS) oil incorporated into a prepared paste with different concentrations and compare it with casein phosphopeptide-amorphous calcium fluoride phosphate (CPP-ACPF) on enamel caries induced on debonded enamel surfaces. Materials and Methods: Forty-four sound permanent premolars were gathered for this study. After mounting, bracket bonding, and then debonding, the teeth were arbitrarily divided into four groups. The artificial enamel caries was created by immersing the samples in a demineralization solution. The enamel surface treated with: Group I (n=11), Control negative (artificial saliva), Group II (n=11), CPP-ACFP paste. Group III(n=11) 5% GS (paste1). Group IV(n=11) 10 % GS (paste2). 8 specimens were exposed to Surface Micro Hardness test (SMH), and 3 were exposed to Scanning Electron Microscope with Energy Dispersive X-ray Spectroscopy (SEM-EDX) after a period of 30 days of treatment.  Results: The results of SMH exhibited a highly statistically significant difference in surface micro-hardness after treatment between the study groups, and the greatest mean value of surface microhardness was seen in 5 % GS (paste1). EDX results exhibited no statistically significant difference after treatment among the study groups. SEM observations showed that a protective layer was formed on the surface of the treated groups. Conclusion: The grape seed oil used in this study displayed a potential remineralization effect in artificial carious lesions on enamel

References

  1. Berrada G. Dental Enamel: Integrating Mineral Chemistry, Biochemistry, Cell Biology and Genetics. Univ Eur Madrid [Internet]. 2021; Available from: http://hdl.handle.net/20.500.12880/524
  2. Sarafopoulou S, Zafeiriadis A, Tsolakis A. Enamel defects during orthodontic treatment. Balk J Dent Med [Internet]. 2018;22(3):64–73. 3. Naveed N, Sabapathy K. Treatment Of White Spot Lesions Post Fixed Orthodontic Therapy. Eur J Mol Clin Med. 2020;7(8):1824–9. doi:10.2478/bjdm-2018-0012
  3. Gocmen GB, Yanikoglu F, Tagtekin D, Stookey GK, Schemehorn BR, Hayran O. Effectiveness of some herbals on initial enamel caries lesion. Asian Pac J Trop Biomed [Internet]. 2016;6(10):846–50. doi.org/10.1016/j.apjtb.2016.08.005
  4. Grohe B, Mittler S. Advanced non-fluoride approaches to dental enamel remineralization : The next level in enamel repair management. Biomater Biosyst [Internet]. 2021;4:100029. doi.org/10.1016/j.bbiosy.2021.100029
  5. Shuping I, Lei M, Burrow MF, Lo EC man, Chu C hung. Prevention of secondary caries using silver diamine fluoride treatment and casein phosphopeptide-amorphous calcium phosphate modified glass-ionomer cement. J Dent [Internet]. 2016;1–7. doi.org/10.1016/j.jdent.2016.12.001
  6. Martin ME, Grao-cruces E, Millan-linares MC, Paz SM de. Grape ( Vitis vinifera L .) Seed Oil : A Functional Food from the Winemaking Industry. Foods. 2020;9, 1360:1–20. doi:10.3390/foods9101360
  7. Garavaglia J, Markoski MM, Oliveira A, Marcadenti A. Grape Seed Oil Compounds : Biological and Chemical Actions for Health. Nutr Metab Insights. 2016;9:59–64. doi:10.4137/NMI.S32910
  8. Pop A, Iurian SM, Benedec D, Moldovan ML. Research Advances in the Use of Bioactive Compounds from Vitis vinifera By-Products in Oral Care. Antioxidants. 2020;9,502:1–32. doi:10.3390/antiox9060502
  9. Hameed HM, Adel A, Tahlawy E, Saniour SH. Assessment of the Remineralizing Efficacy of Grape Seed Extract vs Sodium Fluoride on Surface and Subsurface Enamel Lesions : An In Vitro Study. J Contemp Dent Pr. 2022;23(12):1237–44. doi:10.5005/jp-journals-10024-3442
  10. Nalbantgil D, Oztoprak MO, Cakan DG, Bozkurt K, Arun T. Prevention of demineralization around orthodontic brackets using two different fluoride varnishes. Eur J Dent. 2013;7(1):41–7. doi:10.1055/s-0039-1698994
  11. Yaseen SN, Taqa AA, Al-khatib AR. The effect of incorporation Nano Cinnamon powder on the shear bond of the orthodontic composite ( an in vitro study ). J Oral Biol Craniofacial Res [Internet]. 2020;10(2):128–34. Available from: doi.org/10.1016/j.jobcr.2020.03.008
  12. Thys DG, Martins FRP, Cardinal L, Ribeiro GLU. In vitro enamel surface roughness analysis of 4 methods for removal of remaining orthodontic adhesive after bracket debonding. Angle Orthod. 2023 Mar 1;93(2):213–21. doi:10.2319/031722-227.1
  13. Altinisik H, Erten H. Evaluation of the activities of toothpastes with different contents in the prevention of enamel demineralization. Curr Res Dent Sci [Internet]. 2022 Mar 17;33(1):27–34. doi:10.54614/CRDS.2022.6199
  14. Hamdi K, Hamama HH, Motawea A, Fawzy A, Mahmoud SH. Remineralization of early enamel lesions with a novel prepared tricalcium silicate paste. Sci Rep [Internet]. 2022;12:9926:1–11. doi.org/10.1038/s41598-022-13608-0
  15. Almarsomy DH, Al-khayat FA, Al-taee LA. The preventive / therapeutic effect of CO 2 laser and MI Paste Plus ® on intact and demineralized enamel against Streptococcus mutans ( In Vitro Study ). Heliyon [Internet]. 2023;9(10):e20310. Available from: doi.org/10.1016/j.heliyon.2023.e20310
  16. Taqa AA, Al-sarraf HA. Artificial Saliva Sorption for Three Different Types of Dental Composite Resin (An In Vitro Study). EC Dent Sci. 2019;18(10):2339–44.
  17. Rashid SM, Qasim AA. Remineralizing Effect of GC Tooth Mousse versus Acidulated Phosphate Fluoride on the Surface Microhardness of the Demineralized Enamel. Iraqi Dent J. 2023;45(2):1–8. doi:10.46466/idj.v45i2.280
  18. Ghaleb L, Ali N, Worafi A, Thawaba A, Abdulqader AA, Alkamel A, et al. Evaluation of enamel surface integrity after orthodontic bracket debonding : comparison of three different system. BMC Oral Health [Internet]. 2024;24:358:1–11. Available from: doi.org/10.1186/s12903-024-04138-4
  19. Tarcísio J, Ferreira L, Borsatto MC, Torres CP, Romano FL, Conceição M, et al. Evaluation of Enamel Roughness in Vitro After Orthodontic Bracket Debonding Using Different Methods of Residual Adhesive Removal. Turk J Orthod. 2020;33(1):43–51. doi:10.5152/TurkJOrthod.2020.19016
  20. Rao D, Panwar S, Kothari N. An in vitro comparative evaluation of casein phosphopeptide-amorphous calcium phosphate fluoride, tricalcium phosphate and grape seed extract on remineralization of artificial caries lesion in primary enamel. J Clin Pediatr Dent 2022. 2022;46(5):72–80. doi:10.22514/jocpd.2022.010
  21. Nagi SM, Hassan SN, El-alim SHA, Elmissiry MM. Remineralization potential of grape seed extract hydrogels on bleached enamel compared to fluoride gel : An in vitro study. J Clin Exp Dent. 2019;11(5):e401-7. doi:10.4317/jced.55556
  22. Reynolds EC. Casein Phosphopeptide-Amorphous Calcium Phosphate: The Scientific Evidence. Adv Dent Res [Internet]. 2009 Aug 7;21(1):25–9. doi:/10.1177/0895937409335619
  23. Sebastian R, Paul ST, Azher U, Reddy D. Comparison of Remineralization Potential of Casein Phosphopeptide : Amorphous Calcium Phosphate , Nano- hydroxyapatite and Calcium Sucrose Phosphate on Artificial Enamel Lesions : An In Vitro Study. Int J Clin Pediatr Dent. 2022;15(1):69–73. doi:10.5005/jp-journals-10005-2339
  24. Mirkarimi M, Eskandarion S, Bargrizan M, Delazar A KM. Remineralization of artificial caries in primary teeth by grape seed extract: An in vitro study. J Dent Res Dent Clin Dent Prospect. 2013;7:206–10. doi:10.5681/joddd.2013.033
  25. Açil Y, Mobasseri AE, Warnke PH, Terheyden H, Wiltfang J SI. Detection of mature collagen in human dental enamel. Cal- cif Tissue Int. 2005;76:121–6. doi:10.1007/s00223-004-0122-0

Authors

Noor N. Agha

Ministry of Health/ Nineveh Health Directorate, Mosul, Iraq

Aisha A. Qasim

Department of P.O.P, College of Dentistry, University of Mosul, Mosul, Iraq

Ali R. Al-Khatib

Department of P.O.P, College of Dentistry, University of Mosul, Mosul, Iraq

Rozita Hassan

Orthodontic Unit School of Dental Sciences, Universiti Sains Malaysia Kota Bharu, Kelantan, Malaysia

Identifiers

DOI: 10.33899/rdentj.v26i1.60477

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