Apatite Forming Ability and Gingival Marginal Adaptation of Two Bioactive Bulkfill Composite Restorative Materials (An in vitro comparative study)

Section: Research Paper

Abstract

Aims: The present study aimed to assess the apatite-forming ability and marginal adaptation of two bioactive bulkfill restorative materials in Cl. II cavities after storage in phosphate-buffered saline (PBS). Materials and Methods: Sixteen sound molars with nearly equivalent occlusal size were molded into a PVC tube, then divided into four groups (n=4) based on composite material types. Each tooth received two separate MO and DO cavity preparations, cavity restorations, and thermocycling, then stored for 28 days in PBS. After that, all the teeth samples were sent for FESEM/EDX analyses. Statistical analyses were performed using One-Way ANOVA and Duncan's Multiple Range test to evaluate and compare the results at a 5% significant level. Result: According to the study, there was a statistically significant difference at (P≤0.05) among the groups in terms of gap width formation in µm at restorative materials-gingival enamel margin interfaces. Group B: Ever X Posterior represented the highest mean of gap width (13.05 ± 3.68), followed by group D: Predicta bioactive (7.86 ± 1.22) and group C: Cention N (7.50 ± 1.09), while group A: Tetric powerFill represented the lowest mean of gap width (6.50 ± 1.61). The FESEM images for bioactive materials represented the apatite deposition, and these results were confirmed with the EDX compositional elements analytical results. Conclusion: The gingival marginal adaptation of Cl. II cavities can be affected by the composition and the properties of the composite materials used. The bioactive restorative materials are considered promising materials with clinical beneficial effect.

References

  1. Loguercio, A.D., Rezende, M., Gutierrez, M.F., Costa, T.F., Armas-Vega, A., Reis, A., 2019. Randomized 36-month follow-up of posterior bulk-filled resin composite restorations. J Dent. (2019); 85: 93-102. doi: 10.1016/j.jdent.2019.05.018
  2. Arbildo-Vega, H.I., Lapinska, B., Panda, S., Lamas-Lara, C., Khan, A.S., Lukomska- Szymanska, M. Clinical effectiveness of bulk-fill and conventional resin composite restorations: systematic review and meta-analysis. Polymers (Basel). (2020); 12. doi: 10.3390/polym12081786
  3. Kaisarly, D., El Gezawi, M., Keßler, A., R¨osch, P., Kunzelmann, K.-H., 2020. Shrinkage vectors in flowable bulk-fill and conventional composites: bulk versus incremental application. Clin Oral Invest. (2020); 25(3):1127-1139. doi: 10.1007/s00784-020-03412-3
  4. Hayashi, J., Tagami, J., Chan, D., Sadr A. New bulk-fill composite system with high irradiance light polymerization. Dent Mater. (2020); 36(12), 1615-1623. doi: 10.1016/j.dental.2020.10.012
  5. Zhang, K., Zhang, N., Weir, M.D., Reynolds, M.A., Bai, Y., and Xu, H.H.K. Bioactive dental composites and bonding agents having remineralizing and antibacterial characteristics. HHS Public Access. (2018); 61(4): 669-687. doi: 10.1016/j.cden.2017.05.002
  6. Aziz, N. A., Ranjini, M. A., Kavoor, S., Patel, S., Parvathy, P., Nadig, R. R. Evaluation of microleakage in cervically placed class II restoration with an alkasite restorative material and bulk fill composite resin using confocal laser scanning microscope - an in-vitro study. Inter J Esth Rest Dent. (2022); 3(1): 29-45. doi: 10.56501/intjesthresdent.2022.104
  7. Gamarra, V. S. S., Borges, G. A., Júnior, L. H. B., and Spohr, A. M. Marginal adaptation and microleakage of a bulk-fill composite resin photopolymerized with different techniques. Odonto. (2018); 106(1): 56-63. doi: 10.1007/s10266-017-0294-5
  8. Sadeghi, M. An in vitro microleakage study of class V cavities restored with a new self-adhesive flowable composite resin versus different flowable materials. Dent Res J (Isfahan). (2012); 9(4): 460-465.
  9. Pecie, R., Onisor, I., Krejei, I., Bortolotto, T. Marginal adaptation of direct class II composite restorations with different cavity liners. Oper Dent. (2013); 38:1-11. doi: 10.2341/12-229-L
  10. Bakhsh, T., A., Sadr, A., Shimada, Y., Turkistani, A., Abuljadayel, R., and Tagami, J. Does lining class-II cavities with flowable composite improve he interfacial adaptation?. J Adhes Sci Tech. (2019); 1-17. doi: 10.1080/01694243.2019.1676589
  11. Owens, B.M., Phenbus, J. G., and Johnson, W.W. Evaluation of the marginal integrity of bioactive restorative material. Gen Dent. (2018); 66(3):32-36.
  12. Ladino, L., Bernal, A., Calderón, D., and Cortés, D. “Bioactive Materials in Restorative Dentistry: A Literature Review”. SVOA Dentistry. (2021); 2(2): 74-81.
  13. Omar, A., Aljunayh, M.S., Alharbi, A.G., Aldosari, A.O., Alghamdi, M.M., Alqahtani, N.M., and Elmarakby, A. M. Review Article One Bulk Fill Composite Restorative Material: Advantages and Application Technique. EC DENTAL SCIENCE. (2020); 68(1): 1065-1072.
  14. Van Ende, A., De Munck, J., Lise, D.P., and Van Meerbeek, B. Bulk-Fill Composites: A Review of the Current Literature. J Adhes Dent. (2017); 19(2): 95-110. doi: 10.3290/j.jad.a38141
  15. Francois, P., Fouquet, V., Attal, J., and Dursun, E.. Commercially Available Fluoride-Releasing Restorative Materials : A Review and a Proposal for Classification. Materials. (2020);13(10):2313. doi: 10.3390/ma13102313
  16. Abdallah, A. Elemental and Micromorphological Analysis of New Alkasite Based Restorative Material/Tooth Interface. EDJ. (2022); 68(1): 1065-1072. doi: 10.21608/edj.2021.105950.1866
  17. Jumaah, S.S., Al-Shamma, A.M. Immediate and Long Term Gingival Marginal Leakage of Two Bioactive Bulk Fill Restorative Materials (A Comparative in vitro Study). J Res Med Dent Sci. (2021); 9(7): 120-126.
  18. Angadala, P., Mandava, J., Ravi, R., Hanumanthu, K.R., Penmatsa, P., and Pulidindi, H. An in vitro micro-CT assessment of bioactive restorative materials interfacial adaptation to dentin. Dent Res J. (2022); 19(1): 56-64. doi: 10.4103/1735-3327.351345
  19. Han, S.H., Park, S.H. Comparison of internal adaptation in class II bulk- fill composite restorations using micro-CT. Oper Dent. (2017); 42(2): 203-14. doi: 10.2341/16-303-L
  20. García Marí, L., Climent Gil, A., and Llena Puy, C. In vitro evaluation of microleakage in class II composite restorations: High-viscosity bulk-fill vs conventional composites. Dent Mate J. (2019); 38(5): 721-727. doi: 10.4012/dmj.2018-160
  21. Putignano, A., Tosco, V., Monterubbianesi, R., Vitiello, F., Gatto, M.L., Furlani, M., Giuliani, A., and Orsini, G. Comparison of three different bulk-filling techniques for restoring class II cavities: μCT, SEM-EDS combined analyses for margins and internal fit assessments. J Mech Behav Biomed Mater. (2021); 124: 1-9. doi: 10.1016/j.jmbbm.2021.104812
  22. International Organization for Standardization ISO/ TS 11405: (2015). Dentistry_ Testing of adhesion to tooth structure. 3rd ed. Geneva: International Organization for Standardization.
  23. Assiri, A., Alomairy, A., Nashaat, M. Marginal Adaptation of Bulk-Fill versus Layered Resin Composite Restorations. Inter J Health Sci Res. (2018); 8(6): 65-74.
  24. Baban, L.M. S. The influence of flowable composite liner on microleakage of class II packable composite resin restoration with different application techniques ( Comparative study ). J Bagh Coll Dent. (2019); 24(4): 19-24.
  25. Chiang, Y. C., Rösch, p., Dabanoglu, A., Lin, C. P., Hickel, R., Kunzelmann K. H. Polymerization composite shrinkage evaluation with 3D deformation analysis from microCT images. Dent Mater. (2010); 26: 223-231. doi: 10.1016/j.dental.2009.09.013
  26. Fronza, B.M., Rueggeberg, F.A., Braga, R.R., Mogilevych, B., Soares, L.E.S., Martin, A.A., Ambrosano, G., Giannini, M. Monomer conversion, microhardness, internal marginal adaptation, and shrinkage stress of bulk-fill resin composites. Dent Mater. (2015); 31: 1542-1551. doi: 10.1016/j.dental.2015.10.001
  27. Kaisarly, D., El Gezawi, M., Nyamaa, I., Rosch, P., and Kunzelmann, K. Effects of boundary condition on shrinkage vectors of flowable composite in experimental cavity models made of substrates. Clin Oral Invest. (2019); 23:2403-2411. doi: 10.1007/s00784-018-2706-5
  28. Turkistani, A., Nasir, A., Merdad, Y., Jamleh, A., Alshouibi, E., Sadr, A., Tagami, J., and Bakhsh, T. A. Evaluation of microleakage in class-II bulk-fill composite restorations. J Dent Sci. (2020); 15(4): 486-492. doi: 10.1016/j.jds.2020.01.007
  29. Sardana, A., Kumar, M., and Taneja, S. Comparative evaluation of microleakage and hardness of newer posterior restorative materials. J O Bio Cranio Res. (2022); 12(5): 733-736. doi: 10.1016/j.jobcr.2022.06.006
  30. Bonilla, E.D., Stevenson, R.G., Caputo, A.A., and White, S. N. Microleakage resistance of minimally invasive Class I flowable composite restorations. Oper Dent. (2012); 37(3): 290-298. doi: 10.2341/11-117-L
  31. Shahidi, S., Krejci, I., Dietschi, D. In vitro Evaluation of marginal adaptation of direct class II composite restorations made of different "low-shrinkage" systems. Oper Dent. (2017); 42(3): 273-283. doi: 10.2341/16-096-L
  32. Rizzante, F., Sedky, R., Furuse, A., Teich, S., and Mendonca, G. Validation of a method of quantifying 3D leakage in dental restorations. J of Prosthet Dent. (2020); 123(6): 839-844. doi: 10.1016/j.prosdent.2019.05.020
  33. Cayo-Rojas, C.F., Hernández-Caba, K.K., Aliaga-Mariñas, A.S., Ladera-Castañeda, M. I., and Cervantes-Ganoza, L.A. Microleakage in class II restorations of two bulk fill resin composites and a conventional nanohybrid resin composite: an in vitro study at 10,000 thermocycles. BMC Oral Health. (2021); 21(1): 1-8. doi: 10.1186/s12903-021-01711-8
  34. Zavattini, A., Mancini, M., Higginson, J., Foschi, F., Pasguantonio, G., and Mangani, F. Micro-computed tomography evaluation of microleakage of class II composite restoration: An in vitro study. Europ J Dent. (2018). 12(3): 369-374. doi: 10.4103/ejd.ejd_52_18
  35. Chisnoiu, A. M., Moldovan, M., Sarosi, C., Chisnoiu, R. M., Rotaru, D. I., Delean, A. G., Pastrav, O., Muntean, A., Petean, I., Tudoran, L. B., and Pastrav, M. Marginal adaptation assessment for two composite layering techniques using dye penetration, AFM, SEM and FTIR: An in-vitro comparative study. Appl Sci (Switzerland). (2021); 11(12): 1-14. doi: 10.3390/app11125531
  36. Benetti, A. R., Michou, S., Larsen, L., Peutzfeldt, A., Pallesen, U., and van Dijken, J. W. V. Adhesion and marginal adaptation of a claimed bioactive, restorative material. Biomater Invest in Den. (2019); 6(1): 90-98. doi: 10.1080/26415275.2019.1668131
  37. Fahmy, M. M., Moussa, T. M., Abdelraouf, R. M. Evaluation of ion release, apatite formation and tooth-restoration interface of bioactive resin composite versus conventional resin composite (an in vitro study). EDJ. (2021); 67(2): 1463-1473. doi: 10.21608/edj.2020.46515.1119
  38. Hamdy, T.M. Bioactivity: A new buzz in dental materials. EC Dent Sci. (2018); 17(8): 1278-1283.
  39. Peutzfeldt, A., Mühlebach, S., Lussi, A., Flury, S. Marginal gap formation in approximal “bulk fill” resin composite restorations after artificial ageing. Oper Dent. (2018); 43(2): 180-189. doi: 10.2341/17-017-L
  40. Kaisarly, D., Meierhofer, D., El Gezawi, M., Rösch, P., and Kunzelmann, K.H. Effects of flowable liners on the shrinkage vectors of bulk-fill composites. Clin Oral Invest. (2021); 25(8): 4927-4940. doi: 10.1007/s00784-021-03824-1
  41. Tosco, V., Vitiello, F., Furlani, M., Gatto, M.L., Monterubbianesi, R., Giuliani, A., Orsini, G., Putignano, A. Microleakage Analysis of Different Bulk-Filling Techniques for Class II Restorations: µ-CT, SEM and EDS Evaluations. Materials.( 2021); 14(31): 1-13. doi: 10.3390/ma14317431
  42. Darabi, F., Tayefeh-Davalloo, R., Tavangar, S. M., Naser-Alavi, F., and Boorboo-Shirazi, M. The effect of composite resin preheating on marginal adaptation of class II restorations. J Clin Expe Dent. (2020); 12(7): e68-e687. doi: 10.4317/jced.56869
  43. Delgado, A. J., Ritter, A. V., Donovan, T. E., Ziemiecki, T., and Heymann, H. O. Effect of Finishing Techniques on the Marginal Integrity of Resin-Based Composite and Resin-Modified Glass Ionomer Restoration. J Esthet Rest Dent. (2015); 27(4):184-193. doi: 10.1111/jerd.12130
  44. Park, K. J., Pfeffer, M., Näke, T., Schneider, H., Ziebolz, D., and Haak, R. Evaluation of low-viscosity bulk-fill composites regarding marginal and internal adaptation. Odont. (2021); 109(1): 139-148. doi: 10.1007/s10266-020-00539-2
  45. Hirata, R., Pacheco, R.R., Caceres, E., Janal, M.N., Romero, M.F., Giannini, M., Coelho, P.G., Rueggeberg, F.A. Effect of sonic resin composite delivery on void formation assessed by micro-computed tomography. Oper Dent. (2018); 43: 144-150. doi: 10.2341/16-360-L
  46. Díaz, C.A.P., Shimokawa, C., Sampaio, C.S., Freitas, A.Z., Turbino, M.L. Characterization and comparative analysis of voids in class II composite resin restorations by optical coherence tomography. Oper Dent. (2020). 45: 71-79. doi: 10.2341/18-251-L
  47. Agarwal, R.S., Hiremath, H., Agarwal, J., Garg, A. Evaluation of cervical marginal and internal adaptation using newer bulk fill composites: an in vitro study. J Conserv Dent. (2015); 18: 56-61. doi: 10.4103/0972-0707.148886
  48. Haidy N S, Sherif M, Hefnawy, Shaymaa M N. Degree of conversion and polymerization shrinkage of low shrinkage bulk fill resin composites. Contemp Clin Dent. (2019); 10:465-470 doi: 10.4103/ccd.ccd_332_18
  49. Negovetic Mandic, V., Par, M., Marovic, D., Rakić, M., Tarle, Z., and KlarićSever, E. Blue Laser for Polymerization of Bulk-Fill Composites: Influence on Polymerization Kinetics. Nanomaterials. (2023); 13(2): 1-12. doi: 10.3390/nano13020287
  50. Watts, D. C., and Algamaiah, H. Characterizing surface viscoelastic integrity of ultra-fast photo-polymerized composites: Methods development. Dent Mater. (2020); 36(10): 1255-1265. doi: 10.1016/j.dental.2020.07.008
  51. Par, M., Spanovic, N., Marovic, D., Attin, T., Tarle, Z., Tauböck, T.T. Rapid high-intensity light-curing of bulk-fill composites: A quantitative analysis of marginal integrity. J Dent. (2021); 111: 1-20. doi: 10.1016/j.jdent.2021.103724
  52. Papadogiannis, D., Tolidis, K., Gerasimou, P., Lakes, R., and Papadogiannis, Y. Viscoelastic properties, creep behavior and degree of conversion of bulk fill composite resins. Dent Mater. (2015); 31(12): 1533-1541.DOI: 10.1016/j.dental.2015.09.012
  53. Tod, J.C. Scientific documentation: Cention N. Ivoclar-Vivadent Press: Schaan, Liechtenstein. (2016); Pp:1-5.
  54. Batra, D., Kaur, M., Singh Mann, N., and Jhamb, A. A comparative evaluation of compressive strength of Cention N with glass Ionomer cement: An in-vitro study. Int J Appl Dent Sci. (2019); 5(1): 5-9.
  55. Adsul, P.S., Dhawan, P., Tuli, A., Khanduri, N., and Singh, A. Evaluation and Comparison of Physical Properties of Cention N with Other Restorative Materials in Artificial Saliva: An In Vitro Study. Int J Clin Ped Dent. (2022). 15(3): 350-355. doi: 10.5005/jp-journals-10005-2330
  56. Samanta, S., Das, U.K., Mitra, A. Comparison of microleakage in Class V cavity restored with flowable composite resin, glass ionomer cement and Cention N. Imp J Interdiscip Res. (2017); 3(8):180-183.
  57. Ferracane, J. L., and Lawson, N. C. Probing the hierarchy of evidence to identify the best strategy for placing class II dental composite restorations using current materials. J Esthet Restor Dent. (2021); 33(1): 39-50. doi: 10.1111/jerd.12654
  58. Han, S.H., Sadr, A., Tagami, J., Park, S. H. Internal adaptation of resin composites at two configurations: Influence of polymerization shrinkage and stress. Dent Mater. (2016); 32: 1085-1094. doi: 10.1016/j.dental.2016.06.012
  59. Spagnuolo, G. Bioactive Dental Materials: The Current Status. Materials. (2022); 15(6): 1-3. doi: 10.3390/ma15062065
  60. Roman, A., Stratul, S. I., Rusu, D., Boariu, M., Soanca, A., Balazsi, R., Suciu, M., Moldovan, M., and Bulboacă, A. E. Investigations on the adhesion of new composites for restoring cervical lesions using energy dispersive X-ray analysis and scanning electron microscopy. Scientific Reports. (2019); 9(1): 1-10.
  61. Ciobanu, G., Carja, G., Ciobanu, O., Sandu, I., and Sandu, A. SEM and EDX studies of bioactive hydroxyapatite coatings on titanium implants. Micron. (2009); 40(1): 143-146. doi: 10.1016/j.micron.2008.05.002
  62. Sacher, E., and Franca, R. Surface analysis techniques for dental materials. Dent Bio Mater. (2018); 1-31.
  63. Shafqat, Z., Munir, N., Inayat, N., Khan, M.A., Fareed, M.A., Zafar, M.S. Calicium phosphate-Loaded Novel Polypropelene Glycol-Based Dental Resin Composites: Evaluation of In Vitro Bioactivity. J Compos Sci. (2023); 7:140. doi: 10.3390/jcs7040140
  64. Senra MR, de Lima RB, Souza DDHS, Marquues MDFV, Monteiro SN. Thermal characterization of hydroxyapatite or carbonated hydroxyapatite hybrid composites with distinguished collagens for bone grafts. J Mater Res Technol. (2020); 9: 7190-7200. doi:10.1016/j.jmrt.2020.04.079
  65. Kasraei, S.H., Haghi, S., Valizadeh, S., Panahandeh, N., Nejadkarimi, S. Phosphate Ion Release and Alkalizing Potential of Three Bioactive Dental Materials in Comparison with Composite Resin. Inter J Dent. (2021); 10: 1-8.
  66. Braga, R.R. Calcium phosphates as ion-releasing fillers in restorative resin-based materials. Dent Mater. (2019); 35(1): 3-14. doi: 10.1016/j.dental.2018.10.018
  67. Par, M., Attin, T., Tarle, Z., and Tauböck, T. T. “A new customized bioactive glass filler to functionalize resin composites: acid-neutralizing capability, degree of conversion, and apatite precipitation. J Clin Med. (2020); 9(4): 1173. doi: 10.3390/jcm9041173
  68. Tiskaya, M., Al-Eesa, N., Wong, F., Hill, R. Characterization of the bioactivity of two commercial composites. Dent Mater. (2019); 35(12):1757-1768. doi: 10.1016/j.dental.2019.09.004
  69. Kim, H.M., Himeno, T., Kokubo, T., Nakamura, T. Process and kinetics of bonelike apatite formation on sintered hydroxyapatite in a simulated body fluid. Biomaterials. (2005); 26:4366-73.
  70. Poorni, S., Kumar, R.A., Shankar, P., Indira, R., and Ramachandran, S. Effect of 10% sodium ascorbate on the calcium: Phosphorus ratio of enamel bleached with 35% hydrogen peroxide: an in vitro quantitative energy-dispersive X-ray analysis. Contemp Clin Dent. (2010); 1(4): 223-226. doi. 10.4103/0976-237X.76388
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