Assessment of Blood Response to Serratiopeptidase and Chymotrypsin Therapy on Facial Skin Model of Hyaluronic Acid Injection in Rabbits

Section: Research Paper
Issue
Vol. 26 No. 1 (2026): Volume 26 Issue 1
Pages
221-234

Keywords:

Chymotrypsin, Dermal Filler, Hyaluronic Acid, Rabbits, Serratiopeptidase

Abstract

Aims: The present study aimed to investigate the impact of Serratio peptidase and Chymotrypsin therapy on blood responses following the injection of Hyaluronic Acid in a facial skin model of rabbits. This research is motivated by the growing utilization of Hyaluronic Acid in facial aesthetics and the need to evaluate potential interventions to mitigate any adverse effects on blood parameters. Materials and Methods: A total of 35 rabbits were used in the experiment. The negative control group (no treatment), positive control (Hyaluronic Acid injection), Serratiopeptidase, and Chymotrypsin groups (serratiopeptidase and chymotrypsin therapy). Blood samples were collected at predetermined intervals for analysis. The blood serum was analyzed 24 hours and 72 hours after the injection. The primary blood parameters analyzed included Red Blood Cell Count (RBC), White Blood Cell Count (WBC), Platelet Count, and C-Reactive Protein (CRP) levels. Results indicated that both treatments led to anti-inflammatory responses following the HA-induced inflammation. Conclusion: Chymotrypsin elicited a more pronounced body response to induced inflammation compared to serratiopeptidase, and appears to be better suited for cosmetic applications. However, further research evaluating such responses after more than 72 hours is needed to obtain conclusive results.

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Authors

Abdulrahman Zaid Al-Najjar

Department of Dental Basic Science, College of Dentistry, Gilgamesh University, Baghdad. Iraq

Faehaa Azher Al-Mashhadane

Department of Dental Basic Sciences, College of Dentistry, University of Mosul, Mosul, Iraq

Identifiers

DOI: 10.33899/rdentj.v26i1.60536

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